Évaluation de l’ELISA direct en sandwich à base d’anticorps monoclonaux (MSD-ELISA) pour la détection d’antigène du virus de la fièvre aphteuse à l’aide d’échantillons cliniques.
A direct sandwich ELISA (MSD-ELISA) method based on monoclonal antibodies has previously been developed for the detection of foot-and-mouth disease (FMD) viral antigen. Here, we assessed the sensitivity and specificity of two foot-and-mouth disease viral antigen detection MSD-ELISAs and compared them with the conventional indirect sandwich (IS) -ELISA. MSD-ELISA were able to detect antigen in … Lire la suite