| Liste des signes utilises en Biotechnologie |
| + |
| Positive |
| – |
| Negetive |
| % |
| Percent |
| a.a |
| Amino acid |
| 7-AAD |
| 7-Aminoactinomycin |
| ABL |
| Abelson murine leukaemia virus homology gene |
| ALL |
| Acute lymphatic leukemia |
| AML |
| Acute myeloid leukemia |
| AMP |
| Ampicillin |
| Arg |
| Argenin |
| ATP |
| Adenosin-5’-triphosphate |
| attP site |
| Attachement site |
| BCR |
| Breakpoint cluster region |
| BCR/ABL |
| Breakpoint cluster region/ Abelson murine leukaemia virus |
| BM |
| Bone marrow |
| BrdU |
| 5-bromo-2-deoxyuridine |
| BSA |
| Bovine serum albumin |
| °C |
| Celsius |
| Ca |
| 2+ |
| Calcium |
| CC |
| coiled-coil |
| ΔCC |
| Deleted coiled-coil |
| CD |
| Cluster of differentiation |
| cDNA |
| Complementary DNA |
| cm |
| Centimeter |
| CSF |
| Colony stimulating factor |
| CFU |
| Colony-forming unit |
| CFU-GEMM |
| CFU-granulocyte erythroid megacaryocyte macrophage |
| CFU-GM |
| Colony-forming unit granulocyte, macrophage |
| CIP |
| Calf intestinal phosphatase |
| CLL |
| Chronic lymphatic leukaemia |
| CLP |
| Common lymphoid progenitors |
| Page 138 |
| Abbreviations |
| 138 |
| CML |
| Chronic myeloid leukaemia |
| CMP |
| Common myeloid progenitors |
| CMV |
| Cytomegalovirus |
| CR |
| Complete remission |
| CTP |
| Cytoplasmic transduction peptide |
| dATP |
| desoxy Adenintriphosphat |
| dCTP |
| desoxy Cytosintriphosphat |
| ddH2O |
| Double distilled water |
| DEPC |
| Diethylpyrocarbonate |
| dGTP |
| desoxy Guanosintriphosphat |
| DMEM |
| Dulbecco’s Modified Eagle Medium |
| DMSO |
| Dimethyl sulfoxide |
| DSMZ |
| German Collection of Microorganisms and Cell Cultures |
| DNA |
| Desoxyribonucleic acid |
| DNTPs |
| deoxyribonucleotide triphosphate |
| DTT |
| Dithiothreitol (Threo-1,4-Dimercapto-2,3-Butandiol) |
| dTTP |
| desoxy Thymintriphosphat |
| E. coli |
| Escherichia coli |
| EBV |
| Epstein Barr virus |
| ECL |
| Enhanced chemiluminescence |
| EDTA |
| Ethylenediaminetetraacetate |
| EGFR |
| epidermal growth factor receptor |
| eGFP |
| Enhanced green fluorescent protein |
| ERK |
| extracellular signal regulated kinase |
| FAB classifikation |
| French American British classification |
| FACS |
| Fluorescence activated cell sorting |
| Fak |
| Focal adhesion kinase |
| FCS |
| Fetal calf serum |
| FITC |
| Fluorescein Isothiocyanate |
| g |
| Gram |
| Gal |
| Galactose |
| Page 139 |
| Abbreviations |
| 139 |
| GFP |
| Green fluorescent protein |
| Grb-2 |
| Growth factor receptor-bound protein 2 |
| GST |
| Glutathione S-transferase |
| GTP |
| Guanosintriphosphate |
| Gy |
| Gray |
| HEPES |
| 4-2-hydroxyethyl-1-piperazineethanesulfonic acid |
| HIV |
| Human immunodeficiency virus |
| HMW |
| High molecular weight |
| HPLC |
| High performance liquid chromatography |
| HRP |
| Horse radish peroxidase |
| HSCs |
| Hematopoietic stem cells |
| IFN-α |
| Interferon alpha |
| IgG |
| Immunglobulin G |
| IMDM |
| Iscove’s Modified Dulbecco’s Medium |
| IL-3 |
| Interleukin 3 |
| IPTG |
| Isopropyl-1-thio-Beta-D-galactopyranoside |
| ISE |
| Iterative Stochastic Elimination |
| i.v |
| Intravenous |
| Jak-Stat |
| Janus kinase-signal transducers and activators of transcription |
| JNK |
| c-Jun-N-terminal kinase |
| Kb |
| Kilo base pairs |
| kDa |
| Kilodalton(s) |
| LB-Medium |
| Luria-Bertani-Medium |
| ΔLNGFR |
| Low affinity nerve growth factor receptor |
| LSC |
| Leukemic-stem cell |
| LT-HSC |
| Long-term hematopoietic stem cells |
| M |
| Molar |
| MACS |
| Magnetic activated cell sorting |
| MAPK |
| Mitogen activated protein kinase |
| Page 140 |
| Abbreviations |
| 140 |
| M-BCR |
| Major Breakpoint cluster region |
| m-BCR |
| Minor Breakpoint cluster region |
| μ-BCR |
| Third Breakpoint in the BCR gene, downstream of the M-BCR |
| MBP |
| Myristoyl binding pocket |
| mg |
| Milligram |
| Min |
| Minutes |
| ml |
| Milliliter |
| µg |
| Microgram |
| ml |
| Milli Liter |
| μl |
| Microliter |
| μM |
| Micro molar |
| mM |
| Milli Molar |
| MNCs |
| Mononuclear |
| MPD |
| Myeloproliferative diseases |
| MPH-2 |
| Membrane permeable helix-2 |
| MPP |
| Multipotent progenitor cells |
| mSCF |
| Murine stem cell factor |
| N |
| Amino |
| Ng |
| nano gram |
| NK |
| Natural killer |
| NLSs |
| Nuclear localization signal sequences |
| nSSL |
| Sterically stabilized nano liposomes |
| OD |
| Optical density |
| ORF |
| Open reading frame |
| p185 |
| BCR-ABL |
| 185 kDa fusion protein of t(9;22) |
| p210 |
| BCR-ABL |
| 210 kDa fusion protein of t(9;22) |
| PAGE |
| Polyacrylamide gel electrophoresis |
| PBS |
| Phosphate-buffered saline |
| PCR |
| Polymerase chain reaction |
| PE |
| Phycoerythrin |
| Page 141 |
| Abbreviations |
| 141 |
| pH |
| Reverse logarithmic representation of hydrogen ion |
| concentration |
| Ph+ |
| Philadelphia chromosom positive |
| Ph− |
| Philadelphia chromosom negativ |
| PI |
| Propidium iodide |
| PI3K |
| Phosphatidylinositol-3-kinase |
| PLZF |
| Promyelocytic leukemia zinc finger |
| PML |
| Promyelocytic leukemia protein |
| Pmol |
| Pico Molar |
| PT |
| Peptide transduction |
| PTD |
| Peptide transduction domains |
| RAR |
| retinoic acid receptor |
| RNA |
| Ribonucleic acid |
| rmIL-3 |
| Recombinant murine interleukin-3 |
| rmIL-6 |
| Recombinant murine interleukin-3 |
| RNA |
| Ribonucleic acid |
| Rnase |
| Ribonuclease |
| rpm |
| Revolution per minute |
| RPMI-Medium |
| Roswell Park Memorial Institute-Medium |
| RT |
| Reverse Transcriptase |
| Sca-1 |
| Stem cell antigene 1 |
| SCF |
| Stem cell factor |
| SDS |
| sodium dodecyl sulfate |
| SDS-PAGE |
| SDS-Polyacrylamide gel electrophoresis |
| Ser |
| Serin |
| S/T |
| Serine/threonine |
| STAT |
| Signal transducers and activators of transcription |
| ST-HSC |
| Short-term hematopoietic stem cells |
| STI |
| Signaltransductions inhibitor |
| SOS |
| son of sevenless |
| T |
| Translocation |
| Page 142 |
| Abbreviations |
| 142 |
| t(9;22) |
| Translocation of Chromosom 9 and 22 |
| TAE |
| Tris-acetate-EDTA |
| TAT |
| Trans-Activator of Transcription |
| TBS |
| Tris-buffered saline |
| TBST |
| Tris-buffered saline plus Tween 20 |
| TE |
| Tris-EDTA |
| TEMED |
| Tetramethylethylenediamine |
| Thr |
| Threonin |
| TK |
| Tyrosine kinase |
| Tris |
| Tris(hydroxymethyl)aminomethane |
| TSA |
| Trichostatin A |
| Tyr |
| Tyrosin |
| U |
| Enzyme unit |
| UTR |
| Untranslated region |
| UV |
| Ultraviolet |
| V |
| Volt |
| v/v |
| volume per volume |
| w/v |
| weight per volume |
| WBC |
| White blood cell count |
| WT |
| Wildtype |
| XTT |
| Tetrazolium salt |
| Y177 |
| Tyrosine at a.a. position 177 |
| Y177F |
| Tyrosine mutated to phenylalanine at a.a. position 177 |
Intergal Process Bio-electronique de Laboratoire
Fiches Techniques des équipements de laboratoire (Msds)